Pepper's heat shock transcription factor A1d contributes to heat tolerance and seed germination (2023)

Plant Physiology and Biochemistry, 2022

After harvesting, the metabolism ofGynura bicolorCC (G bicolor) is vigorous, resulting in sugar shortages and accumulation of reactive oxygen species (ROS), which exacerbates quality deterioration. 1-Methylcyclopropene (1-MCP) shows a crucial effect in easing the post-harvest metabolism of fruits and vegetables. This investigation aimed to evaluate the effect of 1-MCP on the elimination of ROS and on sucrose metabolism inG bicolor. In this research,G bicolorwas treated with 10 μL L⁻¹1-MCP for 12 h, followed by storage at 20±2°C and 90±5% relative humidity in the dark for 7 days. During storage, 1-MCP effectively inhibited increases in respiration rate, electrolyte leakage, weight loss rate, ROS levels, and membrane lipid oxidation. Furthermore, starch and hexose degradation decreased in the 1-MCP group, as well as sucrose synthesis and catabolism. Correlation analysis indicated that sugar starvation was associated with respiration, regulation of CAT activities, SOD, and enzymes involved in sucrose metabolism were associated with initial levels of hydrogen peroxide storage. In conclusion, 1-MCP delayed the postharvest quality deterioration ofG bicolorrelieving breathing, inducing oxidative stress to increase ROS elimination and inhibit sucrose metabolism.

Horticultural Sciences, Volume 310, 2023, Article 111701

herbaceous peony (Paeonia lactifloraPall.) is a traditional and famous flower in China that prefers cool climates and does not tolerate high temperatures in summer. Mitogen-activated protein kinase (MAPK) is widely involved in plant signal transduction to various environmental stresses. To clarify the role ofPlMAPK1in response to high temperature stress, it was cloned and its open reading frame was 1215 bp, encoding 405 amino acids. Quantitative real-time PCR (qRT-PCR) analysis showed that when the plant was severely damaged by high temperatures, the level of expression ofPlMAPK1it was at its peak. Furthermore, based on a systematic study of exogenous yeast, it was found that the yeast strain that overexpressesPlMAPK1grew better than the control under high temperature stress. Also,PlMAPK1was temporarily silenced by virus-induced gene silencing (VIGS) technology inP. lactiflora. And under high temperature stress, the leaves of the transgenic plants withered significantly, the accumulation of reactive oxygen species increased, and the photosynthetic capacity significantly decreased. These findings suggested thatPlMAPK1may play a positive regulatory role in the response ofP. lactiflorato high temperature stress, establishing a theoretical basis forP. lactifloraHigh temperature tolerant breed.

Environmental and Experimental Botany, Volume 207, 2023, Article 105196

The role of the AcHsfA2–1 heat shock factor on heat tolerance in kiwi plants was investigated. Exposure of plants to high temperatures (50 ℃) for 1 h resulted in high H₂O₂accumulation on the leaves, while 2h at this temperature caused irreversible damage to the leaves.AcHsfA2–1(conta28668) was the only transcription factor in the four major differentially expressed genes (DEGs) that was upregulated by heat shock stress. stable overexpression ofAcHsfA2–1in kiwi plants increased resistance to heat shock stress of 50 ℃ for at least 2 hours. AcHsfA2–1 can bind to promoters of threeAcHsp20s(Conta16656,conta25875yconta11386), which were highly expressed after heat shock, totrans-Activate them more than 50 times. A total of 68 DEGs were upregulated by overexpression ofAcHsfA2–1directly and indirectly, based on the results of multiple tissue comparisons. three moreAcHsp20s, the promoters of another six DEGs could be directlytrans-activated by AcHsfA2–1, related to 'post-translational modification, protein turnover, chaperones' (acc06344,Conta13870,conta26097,Conta18330), zinc metallopeptidase EGY3 (Conta21153) and an uncharacterized protein (Conta31563). Other DEGs, such as BAG domain-containing proteins (conta19817,account 09180), did not respond directly to AcHsfA2–1, suggesting an indirect regulatory mechanism. This study improves the understanding of the heat stress response mechanism and demonstrates the importance of HsfA2 in perennial plants.

Horticultural Sciences, Volume 310, 2023, Article 111710

Abiotic stresses are the main environmental factors that affect crop growth, productivity and quality. The bHLH family is one of the largest families of transcription factors (TF) in plants, playing important roles in many different response pathways to various abiotic stresses, such as water stress, high salinity, low temperature and soil deficiencies. Since the discovery of the bHLH family in plants, many model plant studies have explored the roles and regulatory mechanisms of bHLH family members in the abiotic stress response. In recent years, bHLH TFs have been extensively studied in regulating the abiotic stress response and resistance in horticultural crops. A review of these studies may provide ideas and guidance for studying the abiotic stress response in horticultural crops. Here, we summarize the structure, classification, and regulation of bHLH TFs and focus on the role and regulatory mechanisms of major bHLH TFs in the abiotic stress response of horticultural crops. Several key bHLH proteins that switch on different signaling pathways in the abiotic stress response, such as ICE1, FER, PIF and various bHLH Ib and IVc, have been described in detail. This review will provide a theoretical basis for future studies on the regulation of bHLH TF in the abiotic stress response and in the yield and quality of horticultural crops under stress conditions. It will also provide resources for creating stress-resistant horticultural crops using transgenic technology.

Horticultural Sciences, Volume 310, 2023, Article 111748

The peony is a traditional ornamental flower, but the extremely short flowering period greatly limits its ornamental value. Gene related to flowering timePoFLC178 encoded amino acids were cloned from petals ofpeony on the door'Fengdan' based on complete third-generation transcriptome sequence. Thus, the expression profiles ofPoFLCat different stages of floral development and different tissues were analyzed by quantitative real-time PCR (qRT-PCR). The semi-open phase showed the highest level of relative expression ofPoFLCduring different stages of flower development, while the petal showed the highest relative level of expression ofPoFLCin different fabrics. Subcellular localization analysis presentedPoFLCwas located in the core. The overexpression vector ofPoFLCwas built and₂transgenic generationArabidopsisThey were obtained. The flowering time and flowering time of the transgenic plants were significantly later than the wild type. It is speculated that the formation of the late flowering phenotype in transgenic plantsArabidopsismay be related to the overexpression ofPoFLC. It is expected thatPoFLCmay play an important role in delaying flowering, and the results may provide a theoretical basis for further studies on the role ofPoFLCin the regular flowering period, and will benefit future research on molecular improvement of tree peony.

Horticultural Sciences, Volume 308, 2023, Article 111532

The GRAS genes (Gibberellin Acid Insensitive, GAI Repressor and Scarecrow) are important regulators of plant growth and development, secondary metabolism, stress and signal transduction. carmine radish (horseradish sativaL.) is a well-known container of red radish pigment grown in Fuling, Chongqing City. To investigate the potential roles of GRAS transcription factors in radish, the genetic structure, evolution, and expression patterns of GRAS were extensively analyzed using bioinformatics. A total of 48 candidate GRAS gene family members were identified and comprised 11 groups:LISCL(13), PAT1(10), SHR(4), SINCE(4), RCS(4), SCL4\7(2), SCL3(3), SCL32(3), DLT(2), LAS(1) eNSP2(1). These groups were submitted to phylogenetic analysis. The transcriptome data revealed potentially different functions forRsGRASgenes in different tissues and in response to tolerance to heavy metals. The results showed that 10RsGRASgenes from four subfamilies (PAT1, DELLA, SCR,yLISCL) were high (FPKM > 5) in all analyzed tissues.RSSCL33a-1, RSSCL26, RSRGL3, RSSCL13–2, RSSCL1–2, RSSCL32–2, RSSCL28–1yRSSHR-2were identified with major changes in both radish cultivars under heavy metal (Cd) stress.RsSCL32–2, RSSCL28–1yRsSCL13–2changed significantly in response to various heavy metal stresses in the heavy metal tolerant turnip 'XCB' compared to the heavy metal sensitive turnip 'HX' (carmine radish). This study provides a comprehensive overview of GRAS genes in radish for radish genetic improvements.